The Biology Spot: The Microbiology Classroom
The Biology Classroom --------- Home -------- The Physiology Classroom
|
In-class |
Homework |
Monday, October 27 (day 2)
1) Population Genetics Problems 2) Pool together class results for alu and D17 |
Population
genetics problems due TUES.
|
Tuesday, October 28 (day 3)QUAD POD FOR MICRO 1:1) jellybean genetics 2) DNA fingerprint simulation MICRO 2 ( normal class): 1) wrap-up alu and D17 LABS |
|
|
Wednesday, October 29 (day
4)
No class for micro 1 1) jellybean genetics 2) DNA fingerprint simulation |
|
Thursday, October 30( day 5)
MICRO 1 1) wrap up alu and D17 labs No class for micro 2 |
1)
Human
Polymerse Chain Packet (alu and D17) due Mon. for all micro classes 2)
Jellybean genetics and
DNA fingerprint labs due Mon. for all micro classes 3)
Study for test
|
|
Friday,
October 31 NO SCHOOL : H APPY HALLOWEEN |
STUDY
FOR TEST |
|
Monday,
November 3 (day 6) Review for test |
STUDY
FOR TEST |
|
Tuesday,
November 4 (day 7) TEST
OVER BASIC GENETICS AND BIOTECHNOLOGY |
|
|
Wednesday,
November 5 (day 8) Bacterial Reproduction discussion -conjugation,
transformation and conjugation |
Read
pages (
) and take notes due THURS. for all micro classes |
|
Thursday,
November 6 ( day 9) Begin transformation lab |
|
|
Friday,
November 7 ( day 10) Wrap up transformation lab and bacterial reproduction discussion |
Transformation
lab due MON. for all micro classes |
|
Monday,
November 10( day 1) Part I: Conjugation lab |
|
|
Tuesday,
November 11 (day 2) Part II: Conjugation lab |
|
|
Wednesday,
November 12 ( day 3) QUAD POD FOR MICRO 1: 1) wrap-up conjugation lab 2) enzyme lab set-up 3) make selective and differential agars Micro 2 ( normal class): 1) wrap-up conjugation lab 2)
enzyme discussion |
Conjugation
lab due FRI. (11/14) for micro 1 Due
MON. (11/17) for micro 2 |
|
Thursday,
November 13 ( day 4) No class for micro 1 QUAD POD FOR MICRO 2: 1) enzyme discussion 2) enzyme lab set-up 3) make selective and differential agars |
Enzyme
lab due WED. (11/19) for all micro classes |
|
Friday,
November 14 ( day 5) Micro 1: Observe plates for enzyme lab No
class for micro 2 |
|
Unit 4:
Microbial Genetics and Biotechnology (Ch
8 & 9)
CONCEPT:
BASIC DNA STRUCTURE
-DNA
-RNA
-nucleotide
-deoxyribose
-DNA
polymerase
-5'
to 3' orientation
-purines
( adenine, guanine)
-pyrmidines
(thymine, cytosine)
-base
pairing
-hydrogen
bonding
-phosphates
-double
helix
-antiparallel
-relationship
between nucleotide, gene,
chromosome, protein
-genome
-DNA
replication (helicase, primase, polymerase)
CONCEPT:
BASIC GENETICS
-gene
-allele
-homozygous
-heterozygous
-dominant
-recessive
-introns
-exons
-simple
genetic problems: monhybrid, dihybrid, and codominant crosses
CONCEPT:
POPULATION GENETICS
-genotype
-phenotype
-allele
frequencies (predicted vs observed)
-Hardy-
Weinberg equilibrium/ equation and assumptions
-sources
of genetic variation ( genetic drift (founder effect and bottleneck effect),
gene flow, natural selection, mutations)
-how
to use a micropipette ( guidelines, conversions and settings)
-VNTR,
STR, RFLP, etc.
-human
DNA extraction ( salt, DNA sample, chelex)
-bacterial
DNA extraction
-gel
electrophoresis (process, equipment and how to read a gel)
-PCR
( PCR vs. DNA replication, process,
equipment and materials needed)
(
6 major ingredients: 1) DNA sample, 2) free nucleotides, 3) primer
4)
magnesium chloride, 5) DNA polymerase and 6) buffer)
-DNA
fingerprint/profile
-reading
autoraidogram from Southern Blot
-Southern
Blot