The Biology Spot: 

The Microbiology Classroom 

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Weekly Schedule for Moore’s Microbiology

Week 9: Monday, October 20 to Friday, October 24

Visit our web site: http://members.tripod.com/moorebiology/

In-class

Homework

Monday, October 20 (day 8)

 

Buffy the Vampire Slayer: A Scenario in Biotechnology

 

Buffy corrections and story ending due WED. for all micro classes

Tuesday, October 21

NO CLASSES: PARENT-TEACHER CONFERENCES

 

Wednesday, October 22 (day 9)

 

Fishing in the Gene Pool activity

-introduction to patterns of inheritance in genetic problems

 

DNA and you packet due THURS. for all micro

Thursday, October 23 (day 10)

 

Monohybrid and Dihybrid genetic problems

Monohybrid and dihybrid genetic problems due FRI. for all micro

Friday, October 25 (day 1)

 

Introduction to Population Genetics discussion and problems

 

 

 

 

 

  Quote of the week:  Curiosity is the wick in the candle of the learning. -William A. Ward

 

Question of the week:   What causes formaldehyde contamination in homes?

 

Unit 4: Microbial Genetics and Biotechnology  (Ch 8 & 9)

CONCEPT: BASIC DNA STRUCTURE

-DNA

-RNA

-nucleotide

-deoxyribose

-DNA polymerase

-5' to 3' orientation

-purines ( adenine, guanine)

-pyrmidines (thymine, cytosine)

-base pairing

-hydrogen bonding

-phosphates

-double helix

-antiparallel

-relationship between  nucleotide, gene, chromosome, protein

-genome

-DNA replication (helicase, primase, polymerase)

 

CONCEPT: BASIC GENETICS

-gene

-allele

-homozygous

-heterozygous

-dominant

-recessive

-introns

-exons

-simple genetic problems: monhybrid, dihybrid, and codominant crosses 

 

CONCEPT: POPULATION GENETICS

-genotype

-phenotype

-allele frequencies (predicted vs observed)

-Hardy- Weinberg equilibrium/ equation and assumptions

-sources of genetic variation ( genetic drift (founder effect and bottleneck effect), gene flow, natural selection, mutations)

 

CONCEPT: BIOTECHNOLOGY

-how to use a micropipette ( guidelines, conversions and settings)

-VNTR, STR, RFLP, etc.

-human DNA extraction ( salt, DNA sample, chelex)

-bacterial DNA extraction

-gel electrophoresis (process, equipment and how to read a gel)

-PCR ( PCR vs. DNA replication,  process, equipment and materials needed)

( 6 major ingredients: 1) DNA sample, 2) free nucleotides, 3) primer

4) magnesium chloride, 5) DNA polymerase and 6) buffer)

-DNA fingerprint/profile

-reading autoraidogram from Southern Blot

-Southern Blot