The Biology Spot: The Microbiology Classroom
The Biology Classroom --------- Home -------- The Physiology Classroom
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In-class |
Homework |
Monday, October 13 ( no school/Columbus day) |
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Tuesday, October 14 ( day 4) NO CLASS FOR MICRO 1 QUAD POD FOR MICRO 2 1) gel electrophoresis of alu loci and photograph 2) analysis of gel 3) streak plate skin sample onto mannitol salt agar 4) continue PCR discussion 5) wrap-up “Cracking the code” video |
Run
through Biointeractive tutorial web-site and write your own detailed
study guide BioInteractive:
Howard Hughes Medical Institute http://www.biointeractive.org/ *click
on VIRTUAL BACTERIA ID LAB: walks through every step of DNA extraction,
PCR, and sequencing DUE
WED. (10/15) for MICRO 1 Lab
8 and 9 due Friday for all micro classes |
Wednesday, October 15 ( day 5)NO CLASS FOR MICRO 2 For micro 1 1) steak plate mannitol salt agar 2) wrap-up “Cracking the code” video |
Lab
8 and 9 due Friday for all micro classes |
Thursday, October 16 (day 6) For all micro classes 1) Staphylococci resistance to antibiotics mini-lab 2) lab 10: preparation of DNA sample for D17 loci for PCR |
1)
Lab 8 and 9 due Friday for all micro classes 2)
Lab 10: pre-lab due Friday |
Friday, October 17 ( day 7)For all micro classes 1) Continue lab 10: gel electrophoresis of D17 loci, photograph and analyze 2) Begin Buffy the vampire slayer biotech scenario project |
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Quote
of the week:
"Science allows you to appreciate even more the
beauty of nature"
Question of the week: What causes the Mexican jumping bean to jump?
Answer to last week's question:
Why do leaves turn color in the fall? The cartenoids which are
responsible for fall colors are present in the leaves during the growing season.
However, the colors are eclipsed by the green chlorophyll.
Toward the end of summer, when the chlorophyll production ceases, the
other colors of the cartenoids ( such as yellow, orange, red and purple) become
visible
Unit
4: Microbial Genetics and Biotechnology (Ch
8 & 9)
CONCEPT:
BASIC DNA STRUCTURE
-DNA
-RNA
-nucleotide
-deoxyribose
-DNA polymerase
-5' to 3' orientation
-purines ( adenine, guanine)
-pyrmidines (thymine, cytosine)
-base pairing
-hydrogen bonding
-phosphates
-double helix
-antiparallel
-relationship between nucleotide, gene, chromosome, protein
-genome
-DNA replication (helicase, primase, polymerase)
CONCEPT:
BASIC GENETICS
-gene
-allele
-homozygous
-heterozygous
-dominant
-recessive
-introns
-exons
-simple genetic problems: monhybrid, dihybrid, and codominant crosses
CONCEPT:
POPULATION GENETICS
-genotype
-phenotype
-allele frequencies (predicted vs observed)
-Hardy- Weinberg equilibrium/ equation and assumptions
-sources of genetic variation ( genetic drift (founder effect and bottleneck effect), gene flow, natural selection, mutations)
CONCEPT:
BIOTECHNOLOGY
-how
to use a micropipette ( guidelines, conversions and settings)
-VNTR,
STR, RFLP, etc.
-human DNA extraction ( salt, DNA sample, chelex)
-bacterial DNA extraction
-gel electrophoresis (process, equipment and how to read a gel)
-PCR ( PCR vs. DNA replication, process, equipment and materials needed)
( 6 major ingredients: 1) DNA sample, 2) free nucleotides, 3) primer
4) magnesium chloride, 5) DNA polymerase and 6) buffer)
-DNA fingerprint/profile
-reading autoraidogram from Southern Blot
-Southern
Blot